Thermal system
Single Peltier-based system
Block format
48-well block
Consumables
48-well Eco plates and optical Eco adhesive seals
Accessories
Eco sample loading dock
Sample volumes
5–20µl validated (optimized for standard 20µl protocols)
Average block ramp rate
5.5°C/sec
Temperature range
35–100°C
Temperature uniformity
± 0.1°C
Optical system
LED excitation (452–486 nm and 542–582 nm), four emission filters (505–545 nm, 562–596 nm, 604–644 nm, and 665–705 nm) and CCD camera
Calibrated dyes at shipment
SYBR, FAM, HEX, VIC, ROX, Cy5: factory-calibrated. Additional dyes within wavelength range of Eco filters are supported with no additional calibration required for implementation
Data collection
Data collected in all four filters for all wells regardless of plate setup; plate setup for data analysis can be altered after run completion.
Melt curve analysis supports continuous data acquisition in a single channel, providing increased data point collection and reduced run times
Real-Time PCR run time (40 cycles)
Less than 40 minutes
Electrical
Voltage: 120VAC=10% Nominal current draw: 8A Frequency: 50/60 Hz=1% Peak Power 500VA, typical power is 180VA
Software
Eco System Software supports all chemistries and a variety of applications, including absolute quantification, relative quantification, allelic discrimination, and high resolution melt curve analysis (HRM)
Warranty
12-month warranty (includes parts and labor)
Instrument dimensions
12.6" closed (height) 14.5" open (height) 13.6" (depth) 12.2" (width)
32 cm closed (height) 36.8 cm open (height) 34.5 cm (depth) 31 cm (width)
Weight
30 lbs (13.6 Kg)
Sensitivity
1 copy
Dynamic range
9 logs linear range
Precision
Discriminates 5,000 and 10,000 template copies with 99% confidence