The Eco Real-Time PCR system contains an advanced high-performance optical system that delivers precise and sensitive fluorescence detection, facilitating all four-color multiplex applications. The system is calibrated for use with SYBR, FAM, HEX, VIC, ROX, and Cy5, but can be used with any real-time PCR chemistry.
For excitation, two panels – 48 fixed LEDs each – provide fluorescent dye excitation over a broad spectrum. Each of the 48 wells is individually illuminated to minimize cross-talk between wells. On the detection side, the optical system enables real-time detection of up to four targets in a single reaction. Four emission filters in a linear filter slide and a high-performance CCD camera detect the fluorescence from each sample target at each cycle. This approach prevents any data loss and allows changes to the plate setup and data analysis even after the run is completed. Standard melt curve and HRM analysis protocols support continuous data acquisition in a single dye channel during the melt for increased data collection and reduced run times.