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The Gene Quantification page describes and summarizes all technical aspects involved in quantitative gene expression analysis using Real-Time qPCR and RT-qPCR.
TATAA Biocenter is a commercial research provider that offers commissioned research and training within molecular diagnostics and gene expression analysis using Real-Time PCR and other molecular techniques to quantify nucleic acids.
The Real-Time PCR Data Markup Language (RDML) is a structured and universal data standard for exchanging quantitative PCR (qPCR) data.
The recent American Recovery & Reinvestment Act of 2009 (ARRA) legislation provides an unprecedented level of funding ($8.2 billion in extramural funding) to the NIH to help stimulate the US economy through the support and advancement of scientific research. While NIH Institutes and Centers have broad flexibility to invest in many types of grant programs, they will follow the spirit of the ARRA by funding projects that will stimulate the economy, create or retain jobs, and have the potential for making scientific progress in 2 years.
This site is written for researchers intending to establish SYBR-based Real-Time PCR in their laboratory.
This essential manual presents a comprehensive guide to the most up-to-date technologies and applications, as well as providing an overview of the theory of this increasingly important technique.
PCR and multiplex PCR: guide and troubleshooting
The University of Texas at Tyler Dr. Bextine Molecular Biology Laboratory
Real-Time PCR overview from Dr. Margaret Hunt, University of South Carolina
Real-Time PCR Research
Real-Time PCR M. Tevfik Dorak, MD, PhD
RTPrimerDB is a public database for primer and probe sequences used in Real-Time PCR assays employing popular chemistries to prevent time-consuming primer design and experimental optimization, and to introduce a certain level of uniformity and standardization among different laboratories.
The Quantitative PCR Primer Database (QPPD) provides information about primers and probes that can be used to quantitate human and mouse mRNA by reverse transcription polymerase chain reaction (RT-PCR) assays. All data has been gathered from published articles, cited in PubMed.
qPrimerDepot - A quantitative Real-Time PCR primer database
The miRBase Sequence Database is a searchable database of published miRNA sequences and annotation. The data were previously provided by the miRNA Registry
Ensembl is a joint project between EMBL - EBI and the Wellcome Trust Sanger Institute to develop a software system which produces and maintains automatic annotation on selected eukaryotic genomes.
A division of the Stanford University Libraries, HighWire Press hosts the largest repository of high impact, peer-reviewed content, with 1263 journals and 5,986,705 full text articles from over 140 scholarly publishers.
Established in 1988 as a national resource for molecular biology information, NCBI creates public databases, conducts research in computational biology, develops software tools for analyzing genome data, and disseminates biomedical information - all for the better understanding of molecular processes affectig human health and disease.
The objective of the Database of Genomic Variants is to provide a comprehensive summary of structural variation in the human genome. The Database of Genomic Variants provides a useful catalog of control data for studies aiming to correlate genomic variation with phenotypic data.
RealTimeDesign (RTD) software from Biosearch Technologies is a free, easy to use, yet powerful design application for Real-Time qPCR. RTD offers both Express and Custom Modes to accommodate the different levels of control that assays may require.
Integrated DNA Technologies Advanced Oligo Design and Analysis
Primer3 - PCR primer design tool
MethPrimer is a program for designing bisulfite-conversion-based Methylation PCR Primers. Currently, it can design primers for two types of bisulfite PCR: 1) Methylation-Specific PCR (MSP) and 2) Bisulfite-Sequencing PCR (BSP) or Bisulfite-Restriction PCR.
This group discusses Real-Time quantitative PCR (qPCR). All qPCR instrumentation and qPCR chemistries are discussed.
Molecular Station Real-Time PCR and Quantitative PCR Forum
The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.
e-PCR identifies sequence tagged sites (STSs) within DNA sequences. Using e-PCR, you can search for sub-sequences that closely match the PCR primers and have the correct order, orientation, and spacing.
The program helps to evaluate the quantity of primers and nucleotides for optimal PCR reaction and the necessary number of cycles.
Reverse and/or complement DNA sequences.
The Poland server will calculate the thermal denaturation profile of double-stranded RNA, DNA or RNA/DNA-hybrids based on sequence input and parameter settings in this form.
Meltmap
Multidimensional data analysis of RT-qPCR data.
BestKeeper Software Determination of stable housekeeping genes, differentially regulated target genes and sample integrity.